Dem alyş wiruslarynyň dokuz görnüşi
Haryt ady
HWTS-RT185A-Dem alyş wiruslarynyň dokuz görnüşi, nuklein kislotasyny kesgitlemek toplumy (Floresan PCR)
Epidemiologiýa
Dem alyş ýollarynyň ýokançlygy, islendik jynsda, ýaşda we sebitde bolup bilýän adam keseliniň iň ýaýran görnüşidir we dünýäde keseliň we ölümiň iň möhüm sebäplerinden biridir.[1]. Kliniki taýdan giňden ýaýran dem alyş patogenlerine grip A wirusy (IFV A), B gripp wirusy (IFV B), täze koronawirus (SARS-CoV-2), dem alyş sintisial wirusy, adenowirus, adam metapneumowirus, rinowirus, parainfluenza wirusy (I / II / III) we mikoplazma wirusy degişlidir.[2,3]. Dem alyş ýollarynyň ýokaşmagy netijesinde ýüze çykýan kliniki alamatlar we alamatlar birneme meňzeýär, ýöne dürli patogenlerden döreýän ýokançlygyň dürli bejeriş usullary, bejeriş täsiri we keseliň gidişi bar[4,5]. Häzirki wagtda dem alyş patogenlerini laboratoriýa taýdan kesgitlemegiň esasy usullary: wirusy izolýasiýa etmek, antigen tapmak we nuklein kislotasyny kesgitlemek. Bu toplum, beýleki kliniki we laboratoriýa netijeleri bilen bilelikde, dem alyş wirusynyň ýokaşmagyna diagnoz goýmakda kömek etmek üçin, dem alyş ýokançlygynyň alamatlary we alamatlary bolan adamlarda belli wirus nuklein kislotalaryny kesgitleýär we kesgitleýär.
Kanal
| FAM | MP nuklein kislotasy |
| ROX | Içerki gözegçilik |
Tehniki parametrler
| Saklamak | 2-8 ℃ |
| Saklanyş möhleti | 12 aý |
| Nusga görnüşi | Oropharyngeal swab; Nasofargeal çiş |
| Ct | COVID-9, IFV A, IFVB, RSV, Adv, hMPV, Rhv, PIV, MP Ct≤35 |
| CV | <5.0% |
| LoD | 200 göçürme / ml |
| Aýratynlygy | Haç reaktiwligi: Kit bilen Boka wirusy, Sitomegalowirus, Epşteýn-Barr wirusy, Herpes simplex wirusy, varisella zoster wirusy, Mumps wirusy, Enterovirus, gyzamyk wirusy, adam koronawirusy, SARS Coronavirus, MERS Coronavirus, Rotavirus, Norovirus, Stremyconee Klebsiella pnewmoniýasy, Streptokokk piýogenleri, Legionella, Pnewmospora, Gemofil grippi, Bacillus pertussis, Staphylococcus aureus, Mikobacterium inçekeseli, gonokokk, Candida albicans, Candida glabra, Aspergillus fromigat tüýkülik, Moraxella katarrh, Lactobacillus, Corynebacterium, adam genom DNK. Päsgelçilik synagy: Mukin (60mg / mL), adam gany (50%), beneprin (2mg / mL), gidroksimetazolin (2mg / mL) 2mg / mL), 5% konserwatiw (20mg / mL), beklometazon (20mg / mL), deksametazon (20mg / mL), fluniseton . . |
| Ulanylýan gurallar | I kesgitleýji reagenti ýazmak üçin ulanylýar: Amaly biosistemalar 7500 Real-wagt PCR ulgamlary, QuantStudio®5 Real-wagt PCR ulgamlary, SLAN-96P Real-wagt PCR ulgamlary (Hongshi Medical Technology Co., Ltd.), LineGene 9600 Plus Real-Time PCR kesgitleýiş ulgamlary (FQD-96A, Hangzhou Bioer tehnologiýasy), MA-6000 Hakyky mukdar termiki welosiped (Suzhou Molarray Co., Ltd.), BioRad CFX Real-Time CFR96 II görnüşli kesgitleýji reagent üçin ulanylýar: Jiangsu Makro & Micro-Test Med-Tech Co., Ltd. tarapyndan EudemonTM AIO800 (HWTS-EQ007). |
| Saklamak | 2-8 ℃ |
| Saklanyş möhleti | 12 aý |
| Nusga görnüşi | Oropharyngeal swab; Nasofargeal çiş |
| Ct | COVID-9, IFV A, IFVB, RSV, Adv, hMPV, Rhv, PIV, MP Ct≤35 |
| CV | <5.0% |
| LoD | 200 göçürme / ml |
| Aýratynlygy | Haç reaktiwligi: Kit bilen Boka wirusy, Sitomegalowirus, Epşteýn-Barr wirusy, Herpes simplex wirusy, varisella zoster wirusy, Mumps wirusy, Enterovirus, gyzamyk wirusy, adam koronawirusy, SARS Coronavirus, MERS Coronavirus, Rotavirus, Norovirus, Stremyconee Klebsiella pnewmoniýasy, Streptokokk piýogenleri, Legionella, Pnewmospora, Gemofil grippi, Bacillus pertussis, Staphylococcus aureus, Mikobacterium inçekeseli, gonokokk, Candida albicans, Candida glabra, Aspergillus fromigat tüýkülik, Moraxella katarrh, Lactobacillus, Corynebacterium, adam genom DNK. Päsgelçilik synagy: Mukin (60mg / mL), adam gany (50%), beneprin (2mg / mL), gidroksimetazolin (2mg / mL) 2mg / mL), 5% konserwatiw (20mg / mL), beklometazon (20mg / mL), deksametazon (20mg / mL), fluniseton . . |
| Ulanylýan gurallar | I kesgitleýji reagenti ýazmak üçin ulanylýar: Amaly biosistemalar 7500 Real-wagt PCR ulgamlary, QuantStudio®5 Real-wagt PCR ulgamlary, SLAN-96P Real-wagt PCR ulgamlary (Hongshi Medical Technology Co., Ltd.), LineGene 9600 Plus Real-Time PCR kesgitleýiş ulgamlary (FQD-96A, Hangzhou Bioer tehnologiýasy), MA-6000 Hakyky mukdar termiki welosiped (Suzhou Molarray Co., Ltd.), BioRad CFX Real-Time CFR96 II görnüşli kesgitleýji reagent üçin ulanylýar: Jiangsu Makro & Micro-Test Med-Tech Co., Ltd. tarapyndan EudemonTM AIO800 (HWTS-EQ007). |
Iş akymy
Makro we mikro-synag wirus DNK / RNA toplumy (HWTS-3017) (bu makro we mikro synag awtomatiki ýadro kislotasy ekstraktory (HWTS-3006C, HWTS-3006B)) we Makro we mikro synag wirus DNK / RNA toplumy (HWTS-3017-8) (Eudemon bilen ulanylyp bilner).TM AIO800 (HWTS-EQ007)) Jiangsu Makro & Micro-Test Med-Tech Co., Ltd.
Çykarylan nusganyň göwrümi 200μL, maslahat berilýän göwrümiň mukdary 150μL.
